目的:综合评价血清中核苷酸结合结构域富含亮氨酸重复序列和含热蛋白结构域受体3(nucleotide-binding domain leucine-rich repeat and pyrin domain-containing receptor 3,NLRP3)的表达水平与阿尔茨海默病的关系。

方法:计算机检索The Cochrane Library、PubMed、Embase、Scopus、CINAHL、CBM、VIP、WanFang Data和CNKI数据库，搜集有关血清NLRP3表达水平与阿尔茨海默病关系的病例对照研究,检索时限从建库至2024年8月1日。由2名研究人员独立进行文献筛选、资料提取和纳入研究的偏倚风险评价，而后采用Stata15.1 SE及Review Manager 5.0软件进行Meta分析。

结果:共纳入9篇文献。Meta分析的结果显示:血清NLRP3表达水平与阿尔茨海默病相关SMD=1.66,95%CI=1.28~2.04,P<0.01)。根据NLRP3测量方法的不同进行亚组分析,酶联免疫吸附法组(SMD=1.48,95%CI=1.15~1.80,P<0.01)和PCR组(SMD=2.12,95%CI=0.78~3.45,P=0.002)均显示NLRP3水平与阿尔茨海默病相关。敏感性分析结果显示结果稳定。

结论:高水平的血清NLRP3表达水平可能与阿尔茨海默病的发生有关。受纳入研究质量和数量的限制,上述结论需进一步开展高质量的研究予以验证。

Objective: To systematically review evaluate the association between the expression levelsof NLR family, pyrin domain-containing 3 protein (NLRP3)in serum and Alzheimer'sdisease ( AD ).

Methods:We searched databases including the Cochrane Library, PubMed, Embase,CBM, VIP, Wan Fang Data and CNKl databases committed to case control studies on therelationship between serum NLRP3 level and AD. The retrieval time limit was from theestablishment of the database to August 2024. Two reviewers independently screenedliterature, extracted data and assessed the risk of bias of included studies. Meta analysiswas performed by Stata 15.1 SE and Review Manager 5.0 software.

Results: Nine articles were included in total. The results of meta analysis showed that serum NLRP3 expression level was associated with AD(SMD=1.66,95%CI=1.28-2.04,P<0.01)Subgroup analysis was conducted based on different  NLRP3 measurement methods, and both the enzyme-linked immunosorbent assay group(SMD=1.48,95%CI=15-1.80,P<0.01 and the PCR group(SMD=2.12,95%CI=0.78-3.45,P-0.002)showed an association between NLRP3 levels and AD. Sensitivity analysis showed that the results were stable.

Conclusion: The meta analysis suggested that high level of serum NLRP3 expression maybe associated with the development of AD. Limited by the quality and quantity of includedstudies, the above conclusions needed to be verified by more clinical studies.