Objective: To explore the effectiveness of interactive scalp acupuncture therapy for post-stroke gait in hemiplegia and mobility. Methods: Ninety post-stroke patients with gait in hemiplegia who were sequentially recruited in this study and meeting the inclusion criteria were randomly divided into three groups: interactive scalp acupuncture group (n = 30, interactive scalp acupuncture therapy combined with rehabilitation training at the same time), traditional scalp acupuncture group (n = 30, rehabilitation training performed after scalp acupuncture therapy) and control group (n = 30, only rehabilitation training). All patients were continuously treated for 3 months. The 5-meter turn-back test was applied to evaluate the improvement in gait quality after treatment. The modified Barthel Index was used to evaluate the effect of improvement in mobile ability on comprehensive rehabilitation efficiency. Results: After treatment, the walking time of 5-meter turn-back test and the modified Barthel Index of three groups were both improved as compared with those before treatment (all P < 0.01). The improvement degree in walking time of 5-meter turn-back test of interactive scalp acupuncture group was significantly greater than those of traditional scalp acupuncture group (P < 0.05) and the control group(P < 0.01). Meanwhile, the difference in modified Barthel Index before and after treatment of interactive scalp acupuncture group was significantly greater than that of the control group (P < 0.05), and also greater than that of the traditional scalp acupuncture group but with no statistical significance (P > 0.05). Conclusion: Interactive scalp acupuncture therapy can improve the recovery of post-stroke patients with gait in hemiplegia, thus improve the patients' mobile ability.